Sloning BioTechnology’s proprietary advanced technology, Slonomics®, is a leading platform that we use to manufacture SlonoMax® libraries for protein engineering. Unlike traditional methods which rely on individually designed and synthesized single stranded oligos, which have to be joined (ligated) together, Slonomics® uses a library of pre-made double stranded triplets that act as universal building blocks sufficient for thousands of gene syntheses processes. The triplet library represents all possible sequence combinations necessary to build any desired DNA molecule.

Consisting of double stranded DNA triplets, these standardized building blocks, are combined by means of a series of validated reaction steps (see Figure). This high level of standardization allows for the process to be fully automated on a sophisticated robotic platform, yielding an exceptional level of throughput and consistency. The double stranded structure of the building blocks guarantees a stable and reliable synthesis process, irrespective of the desired sequence.

Slonomics validated reaction steps

In the first phase of Slonomics®, the triplet building blocks are sequentially ligated in a series of cycles referred to as elongation. The output of this elongation phase is a large number of sub-fragments of the target sequence referred to as E-blocks. Together these E-blocks comprise the complete target sequence.

These E-blocks are then assembled in a second synthesis phase, referred to as transposition, to generate the desired DNA. Adjoining E-blocks are ligated together to create “T-blocks” which are then sequentially ligated to generate larger fragments. This process is repeated until all the blocks are combined into a single DNA molecule comprising the complete sequence.

Full length DNA products are then cloned and sequenced. After sequence verification, the cloned products are delivered to the customer.

The modular system of Slonomics® and the fact that it uses highly standardized triplet building blocks means that the genes and mutant libraries it produces offer very significant quality improvements over traditional cloning technologies.
 

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